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SPARCL™[1] – A Simpler and Faster ELISA Alternative

SPARCL™ (Spatial Proximity Analyte Reagent Capture Luminescence) assays use two specific antibodies; one conjugated to HRP, the other to acridan, a chemiluminescent substrate. When HRP and acridan conjugated antibodies bind to their target biomarker they are brought into close proximity. Upon addition of hydrogen peroxide (trigger solution), HRP catalyzes oxidation of proximal acridan molecules causing a flash of chemiluminescence that is proportional to biomarker concentration. Acridan molecules distant from HRP are not oxidized and therefore produce no luminescence. This principle allows development of a range of rapid and sensitive homogeneous immunoassays.

SPARCL™ Kits
– Simple luminescence immunoassays
– Single 30-min incubation
– No wash steps
– High sensitivity
– High throughput
Excellent alternatives to ELISA’s

A typical SPARCL™ assay requires no wash steps, uses a single incubation, and takes approximately 45 minutes start to finish. This contrasts with ELISA’s that require 1-4 incubations, 1-3 wash steps and take 2-4+ hours. SPARCL™ kits require a luminometer capable of simultaneous injection and measurement. Click here to find out if your luminometer is compatible. Click here for answers to SPARCL FAQs.

Currently available SPARCL™ kits are listed below. Click on the catalog numbers for the kit instructions.

A list of publications that have used our SPARCL™ assays can be found at the bottom of this page.

Orders may be placed by phone, fax, email, or via our online shop.

SPARCL™ Assays
Acute Phase Protein SPARCL™ Assays
(96-well)
 Kit Catalog No. Price
 Rat AGP AGP-SP-2 $500
 Chicken AGP AGP-SP-5 $500
 Cat AGP AGP-SP-8 $500
 Cow AGP AGP-SP-11 $400
 Dog Ceruloplasmin CER-SP-4 $450
 Cat Ceruloplasmin CER-SP-8 $500
 Mouse CRP CRP-SP-1 $500
 Rat CRP CRP-SP-2 $500
 Monkey CRP CRP-SP-3 $500
 Dog CRP CRP-SP-4 $500
 Cat CRP CRP-SP-8 $500
 Pig CRP CRP-SP-9 $500
 Rabbit CRP CRP-SP-10 $500
 Cow CRP CRP-SP-11 $500
 Human CRP*
(serum/plasma)
CRP-SP-20 $400
 Human CRP*
(saliva)
CRPS-SP-20 $400
 Rat Haptoglobin HAPT-SP-2 $500
 Monkey Haptoglobin HAPT-SP-3 $500
 Dog Haptoglobin HAPT-SP-4 $500
 Cat Haptoglobin HAPT-SP-8 $500
 Pig Haptoglobin HAPT-SP-9 $500
 Cow Haptoglobin  HAPT-SP-11 $500
 Horse Haptoglobin HAPT-SP-14 $500
 Human Haptoglobin* HAPT-SP-20 $500
 Monkey Lactoferrin LF-SP-3 $450
 Dog Lactoferrin LF-SP-4 $450
 Cow Lactoferrin LF-SP-11 $500
 Human Lactoferrin* LF-SP-20 $450
 Monkey LRG1 LRG-SP-3 $500
 Cat LRG1 LRG-SP-8 $500
 Human LRG1* LRG-SP-20 $500
 Rat Murinoglobulin MUR-SP-2 $500
 Dog Neutrophil Elastase NEL-SP-4 $500
 Human Neutrophil Elastase NEL-SP-20 $500
 Dog Procalcitonin PCT-SP-4 $500
 Monkey SAA SAA-SP-3 $500
 Dog SAA SAA-SP-4 $500
Chicken SAA SAA-SP-5 $500
 Cat SAA SAA-SP-8 $500
 Cow SAA SAA-SP-11 $500
 Horse SAA SAA-SP-14 $500
 Human SAA* SAA-SP-20 $500
 Monkey Transthyretin TTR-SP-3 $500
  *For research use only
Cardiovascular SPARCL™ Assays
(96-well)
 Human H-FABP HFABP-SP-20 $450
 Mouse Myoglobin MYO-SP-1 $500
 Rat Myoglobin MYO-SP-2 $500
 Monkey Myoglobin MYO-SP-3 $500
 Pig Myoglobin MYO-SP-9 $500
 Rabbit Myoglobin MYO-SP-10 $500
 Mouse Cardiac Troponin-I CTNI-SP-1 $500
 Rat Cardiac Troponin-I CTNI-SP-2 $500
 Monkey Cardiac Troponin-I CTNI-SP-3 $500
 Dog Cardiac Troponin-I CTNI-SP-4 $500
 Pig Cardiac Troponin-I CTNI-SP-9 $500
 Rabbit Cardiac Troponin-I CTNI-SP-10 $500
Immunoglobulin SPARCL™ Assays
(96-well)
 Mouse IgG IGG-SP-1 $350
 Mouse IgG1 IGG1-SP-1 $350
 Mouse IgG2a IGG2A-SP-1 $350
 Mouse IgG2b IGG2B-SP-1 $350
 Mouse IgG3 IGG3-SP-1 $350
 Mouse IgM IGM-SP-1 $350
 Rat IgA IGA-SP-2 $400
 Rat IgG IGG-SP-2  $350
 Rat IgM IGM-SP-2 $350
 Monkey IgA IGA-SP-3 $500
 Monkey IgE IGE-SP-3 $500
 Monkey IgG IGG-SP-3 $500
 Monkey IgM IGM-SP-3 $500
 Dog IgE IGE-SP-4 $500
 Dog IgG IGG-SP-4 $400
 Dog IgM IGM-SP-4 $400
 Cat IgE IGE-SP-8 $500
 Rabbit IgG IGG-SP-10 $400
 Rabbit IgM IGM-SP-10 $400
 Cow IgA IGA-SP-11 $350
 Cow IgG IGG-SP-11 $350
 Cow IgM IGM-SP-11 $350
 Goat IgG IGG-SP-13 $350
 Goat IgM IGM-SP-13 $350
 Horse IgA IGA-SP-14 $400
 Horse IgG IGG-SP-14 $400
 Horse IgM IGM-SP-14 $400
 Human IgG**
– For PK studies
IGG-SP-20 $500
 **Poster
Kidney Function SPARCL™ Assays
(96 well)
 Rat Cystatin-C CYSC-SP-2 $500
 Monkey Cystatin-C CYSC-SP-3 $500
 Dog Cystatin-C CYSC-SP-4 $500
 Cat Cystatin-C CYSC-SP-8 $500
Immunotoxicity SPARCL™ Assays
(96-well)
 Rat Anti-KLH IgG KLHG-SP-2 $500
 Rat Anti-KLH IgM KLHM-SP-2 $500
 Monkey Anti-KLH IgG KLHG-SP-3 $500
 Monkey Anti-KLH IgM KLHM-SP-3 $500
Skeletal Muscle SPARCL™ Assays
(96-well)
 Multi-Species  Troponin-C STNC-SP $500
Steroid Hormone SPARCL™ Assays
(96-well)
 Cortisol CORT-SP $400
 Testosterone TEST-SP $400
Tumor Biomarker SPARCL™ Assays
(96-well)
  Dog Alpha-fetoprotein AFP-SP-4 $500
  Cat Alfa-fetoprotein AFP-SP-8 $500
  Human CA 19-9 CA19-SP-20 $500
  Human CA 72-4 CA72-SP-20 $500
  Human CYFRA 21-1 CYFR-SP-20 $500
  Dog Thymidine Kinase 1 TK1-SP-4 $500
  Cat Thymidine Kinase 1 TK1-SP-8 $500
  Cow Thymidine Kinase 1 TK1-SP-11 $500
  Human Thymidine Kinase 1 TK1-SP-20 $500
PEG SPARCL™ Assays
(96-well)
 mPEG MPEG-SP $500
 PEG PEG-SP $500
SPARCL™ Reagents & Services
 SPARCL™ Plate (96-well) SP-PL $100
 Conjugate Buffer (25 ml) SPB25-1  $250
 Acridan Conjugation SP-AC $1500 / mg*
 HRP Conjugation SP-HRP $1200 /mg*
*Minimum conjugation = 1 mg

For technical advice please email us at [email protected]. All SPARCL kits listed above are manufactured exclusively by Life Diagnostics, Inc.

SPARCL™ Literature References

  1. Baka R, et al. Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic sezures. J Proteomics 231:1039777 (2021)
  2. Kules J, et al. The plasma proteome and the acute phase protein response in canine pyometra. J Proteomics 223:103817 (2020)
  3. Liu M, et al. Cardiovascular-renal axis disorder and the acute-phase proteins in cats with congestive heart failure caused by primary cardiomyopathy. J Vet Intern Med. 34)3):1078-1090 (2020)
  4. Dalanezi FM, et al. Concentrations of acute phase proteins in milk from cows with clinical mastitis caused by different pathogens. Pathogens 9(9):706 (2020)
  5. Boulay I, et.al. SPARCL™: Use of a novel technology in validation of a Non-human primate and rat cardiac troponin-I assay in serum. CiToxLAB, 2016
  6. Boulay I, et.al. SPARCL™: Use of a Novel Technology in Validation of a Non-Human Primate C-Reactive Protein Assay in Serum. CiToxLAB. 2016

SPARCL™ Case Studies – Third party evaluations of SPARCL™ technology
1. Johnson and Johnson
2. Monsanto
3. Lumigen – Human IgG kit (IGG-SP-20)

[1] The SPARCL™ technology was developed by Lumigen Corp.

Copyright Life Diagnostics 2022

Use Statement: All products are for research use only.
Not intended for any diagnostic or therapeutic purpose.

If you are interested in our Veterinary Diagnostic products, please visit: Vetbiomarkers.com Dismiss