New Products

Dog IgE SPARCL™ Kit – added July 18, 2017
The dog IgE SPARCL kit (IGE-SP-4) uses two of our recently developed monoclonal antibodies (see below). It allows rapid measurement of IgE levels in dog serum and plasma. Pricing can be found on the SPARCL kits page.

Anti-dog IgE monoclonals – added July 17, 2017
We have recently developed two anti-dog IgE monoclonal antibodies: IGE-4-4D1 and IgE-4-13H5. They can be used as a pair to develop ELISA and SPARCL assays for measurement of IgE in dog serum and plasma. The antibodies were developed by Life Diagnostics. We purify them under gentle conditions from serum-free media, avoiding low-pH elution steps that often partially denature antibodies. They are priced at $500/mg.

Monkey Leucine-rich alpha-2-glycoprotein-1 (LRG1) SPARCL™ Kit – added July 10, 2017
LRG1 is a positive acute phase protein that is elevated in human serum during inflammation and infection. It may also be a positive acute phase reactant in monkeys. The monkey LRG1 SPARCL™ kit (LRG-SP-3) allows measurement of LRG1 using a single 30-minute incubation. The kit uses antibodies and monkey LRG1 manufactured by Life Diagnostics. Please refer to  the SPARCL kits webpage for pricing.

Human Leucine-rich alpha-2-glycoprotein-1 (LRG1) SPARCL™ Kit – added June 27, 2017
LRG1 is a positive acute phase protein that is elevated in serum during inflammation and infection. Our human LRG1 SPARCL™ kit (LRG-SP-20) allows measurement of LRG1 using a single 30-minute incubation. Unlike ELISA assays that take much longer, it requires no wash steps. Refer to  the SPARCL kits webpage for pricing.

Cat IgE  SPARCL™ Kit – added May 15, 2017
IgE is a useful biomarker for allergen-related diseases. Our cat IgE SPARCL™ assay (IGE-SP-8) allows rapid (30-min) measurement of IgE in cat serum and plasma. Please refer to our SPARCL™ kits page for pricing.

Cat LRG1 – added May 10, 2017
LRG1 (leucine rich alpha 2 glycoprotein-1) is a glycosylated serum protein. In humans, it is an acute phase protein that is upregulated in various cancers, rheumatoid arthritis and Kawasaki disease. Cat LRG1 (catalog no. 9060) is available in 0.1 mg aliquots, priced at $500. We are currently making antibodies and plan to make SPARCL and ELISA kits for detection of human, cow, cat and dog LRG1.

Human LRG1 – added May 10, 2017
LRG1 (leucine rich alpha 2 glycoprotein-1) is a glycosylated serum protein. In humans, it is an acute phase protein that is upregulated in various cancers, rheumatoid arthritis and Kawasaki disease. Human LRG1 (catalog no. 9050) is available in 0.1 mg and 1 mg aliquots, priced at $250 and $2000 respectively.

Cow LRG1 – added May 5, 2017
LRG1 (leucine rich alpha 2 glycoprotein-1) is a glycosylated serum protein. In humans, it is an acute phase protein that is upregulated in various cancers, rheumatoid arthritis and Kawasaki disease. Cow LRG1 (catalog no. 9040) is available in 0.1 mg and 1 mg aliquots, priced at $250 and $2000 respectively.

Dog LRG1 – added May 4, 2017
LRG1 (leucine rich alpha 2 glycoprotein-1) is a glycosylated serum protein. In humans, it is an acute phase protein that is upregulated in various cancers and diseases. It may serve as a useful biomarker in dogs also. We offer native dog LRG1 (catalog no. 9030) in 0.1 mg and 1 mg aliquots, priced at $250 and $2000 respectively.

Human Lactoferrin SPARCL™ Kit – added April 26, 2017
Lactoferrin is an iron-binding glycoprotein found in milk, serum, saliva and urine. Our human lactoferrin SPARCL™ kit (LF-SP-20) allows measurement of lactoferrin using a single 30-minute incubation. Unlike ELISA assays that take much longer, it requires no wash steps. Refer to  the SPARCL kits webpage for pricing.

Cat Serum Amyloid A (SAA)  SPARCL™ Kit – added April 25, 2017
SAA is a positive acute phase protein in cats. Its serum concentrations can increase >50 fold during inflammation and disease.  Similar to CRP in dogs, SAA serves as an excellent acute phase biomarker in cats (CRP is not an acute phase reactant in cats). Refer to the SPARCL kits webpage for pricing of the cat SAA SPARCL™ kit (SAA-SP-8).

Monkey and Dog Lactoferrin SPARCL™ Kits – added April 17, 2017
Lactoferrin is an iron-binding glycoprotein found in milk and serum. In humans, it is used as a biomarker of IBS. It may prove similarly useful in monkeys and dogs. Pricing of our dog (LF-SP-4) and monkey (LF-SP-3) lactoferrin kits can be found on the SPARCL kits webpage.

Rat Murinoglobulin SPARCL™ Kit – added April 12, 2017
Murinoglobulin is a negative acute phase reactant in rats; serum levels decrease during inflammation and infection. The rat murinoglobulin SPARCL™ kit (MUR-SP-2) allows rapid measurement of murinoglobulin in serum or plasma.  Pricing can be found on our SPARCL kits webpage.

Monkey Transthyretin SPARCL™ Kit – added April 6, 2017
The monkey transthyretin kit (TTR-SP-3) allows rapid measurement of transthyretin (also known as prealbumin) in serum and plasma. Transthyretin is a negative acute phase reactant; serum levels decrease as a result of inflammation and infection. Pricing can be found on our SPARCL kits webpage.

Dog Ceruloplasmin SPARCL™ Kit – added March 24, 2017
The dog ceruloplasmin kit (CER-SP-4) allows measurement of ceruloplasmin in serum and plasma using a rapid, non-enzyamatic, luminescence assay. Pricing can be found on our SPARCL kits webpage. The kit is for research use only.

Rat, Cat, Dog & Monkey Cystatin-C SPARCL™ Kits – added March 13-17, 2017
SPARCL™ assays for rat, cat and dog and monkey cystatin-C (CYSC-SP-2, CYSC-SP-8, CYSC-SP-4 and CYSC-SP-3) are now available. Cystatin-C is a biomarker of kidney function. Prices can be found on our SPARCL kits webpage.

Testosterone SPARCL™ Kit – added January 3, 2017
The testosterone SPARCL™ assay (TEST-SP) is a competitive assay that allows measurement of testosterone in serum, urine and saliva using a single 30-minute incubation step. Pricing can be found on our SPARCL kits webpage. The kit is for research use only.

Human IgG SPARCL™ Kit – added December 5, 2016
The human IgG SPARCL™ kit (IGG-SP-20) is intended for use in PK studies. It allows measurement of human IgG in mouse, rat, dog and monkey serum. Pricing can be found on our SPARCL kits webpage. The kit is for research use only.

Cortisol SPARCL™ Kit – added November 18, 2016
This assay is our first small molecule SPARCL™ assay (CORT-SP). It is a competitive assay. Cortisol in the sample competes for binding of HRP-cortisol to an acridan labelled cortisol antibody. The assay allows measurement of total cortisol in serum, saliva and urine. Pricing can be found on our SPARCL kits webpage. The kit is for research use only.

SPARCL™ Video – added November 4, 2016
Check out our new video that highlights the technology used in our SPARCL™ kits.

Human Haptoglobin SPARCL™ Kit – added October 26, 2016
The kit allows rapid measurement of haptoglobin serum and plasma (HAPT-SP-20). Pricing can be found on our SPARCL kits webpage. The kit is for research use only.

Human Serum Amyloid A (SAA) – added October 22, 2016
Using our recently developed human SAA SPARCL™ kit we screened 90 human acute phase fluid samples and identified units with sufficiently high SAA levels to allow purification. The SAA (catalog# 9006) is highly purified, migrating as a single ~12 kDa band on SDS PAGE. It can be used in western blots, for antibody production, or to prepare assay controls and calibrators. Refer to our acute phase protein page for pricing.

Monkey SAA SPARCL™ Kit – added October 19, 2016
The kit allows rapid measurement of SAA in monkey serum and plasma (SAA-SP-3). Because SAA binds strongly to lipoproteins that interfere with SAA measurement, samples must be briefly treated with dissociation buffer (provided with the kit) prior to assay. Whereas ELISA kits take 2-4 hours, the SPARCL™ assay can be completed in one hour, including the dissociation step. Pricing can be found on our SPARCL kits webpage.

Human SAA SPARCL™ Kit – added October 18, 2016
The kit allows rapid measurement of SAA in serum, plasma or fluid samples (SAA-SP-20). Because SAA binds strongly to lipoproteins that interfere with SAA measurement, samples must be briefly treated with dissociation buffer (provided with the kit) prior to assay. Whereas ELISA kits take 2-4 hours, the SPARCL™ assay can be completed in one hour, including the dissociation step. Pricing can be found on our SPARCL kits webpage. The kit is for research use only.

Cow Lactoferrin SPARCL™ Kit – added October 12, 2016
Lactoferrin is an anti-bacterial protein of ~80 kDa. Its levels increase in serum and milk during infection and disease. It has been reported to be a useful biomarker of mastitis. Kit LF-SP-11 allows rapid measurement of lactoferrin in serum and milk. Refer to the SPARCL kits webpage for procing.

Human CRP SPARCL™ Kits – added October 10, 2016
SPARCL™ kits are available for measurement of C-reactive protein in serum (CRP-SP-20) and saliva (CRPS-SP-20). Whereas ELISA kits take 2-4 hours, require multiple incubations and wash steps, SPARCL™ assays require a single 30-minute incubation and no wash steps. Prices can be found on our SPARCL kits webpage. The kits are for research use only.

Cow Alpha-1-Acid Glycoprotein SPARCL™ Kit – added October 7, 2016
The cow AGP SPARCL kit (AGP-SP-11) allows rapid measurement of AGP, a positive acute phase reactant, in serum, plasma and milk. We found that serum AGP levels increase >8-fold. As a result of mastitis, AGP levels in milk can increase >300-fold. Prices can be found on our SPARCL kits webpage.

Rat Alpha-1-Acid Glycoprotein SPARCL™ Kit – added September 27, 2016
The rat AGP SPARCL kit (AGP-SP-2) allows rapid measurement of AGP, a positive acute phase reactant, in serum and plasma. Prices can be found on our SPARCL kits webpage.

Goat IgG and IgM SPARCL™ Kits – added September 19, 2016
SPARCL™ kits are now available for goat IgG (IGG-SP-13) and IgM (IGM-SP-13). Prices can be found on our SPARCL kits webpage.

Rabbit IgG and IgM SPARCL™ Kits – added September 16, 2016
SPARCL™ kits are now available for rabbit IgG (IGG-SP-10) and IgM (IGM-SP-10). Prices can be found on our SPARCL kits webpage.

Horse IgA, IgG and IgM SPARCL™ Kits – added September 14, 2016
SPARCL™ kits are now available for horse IgA (IGA-SP-14), IgG (IGG-SP-14) and IgM (IGM-SP-14). Prices can be found on our SPARCL kits webpage.

Cow IgG and IgM  SPARCL™ Kits – added September 8, 2016
SPARCL™ kits are now available for cow IgG (IGG-SP-11) and IgM (IGM-SP-11). Pricing can be found on our SPARCL kits webpage.

Cow IgA SPARCL™ Kit – added September 4, 2016
IGA-SP-11. Pricing can be found on our SPARCL kits webpage.

Dog IgM SPARCL™ Kit – added August 17, 2016
IGM-SP-4. Pricing can be found on our SPARCL kits webpage.

Dog Procalcitonin SPARCL™ Kit – added August 12, 2016
Procalcitonin (PCT) is a potential biomarker for sepsis and other bacterial infections in dogs. Our dog PCT SPARCL™ assay (PCT-SP-4) is for research use only. It allows rapid measurement of PCT levels in dog serum. Pricing can be found here.

PEG SPARCL™ Kit – added August 6, 2016
We now manufacture a SPARCL kit (PEG-SP) for detection of PEG and PEGylated proteins. This assay is different than our MPEG-SP kit in that it detects free PEG and PEGylated proteins with different terminal end groups. The assay uses acridan and HRP conjugates of the PEG-backbone specific antibody (1D9-6) developed at Life Diagnostics. The assay uses a rapid (30 min) luminescence format that requires no wash steps. Pricing can be found on our PEG SPARCL™ kits webpage.

Methoxy-PEG SPARCL™ Kit – added July 28, 2016
We now manufacture a SPARCL kit (MPEG-SP) for detection of methoxy-PEG (mPEG) and mPEGylated proteins. The assay uses a methoxy-specific monoclonal antibody conjugated to HRP and a PEG-backbone specific antibody conjugated to acridan. Both antibodies are manufactured exclusively by Life Diagnostics. The assay uses a rapid (30 min) luminescence format that requires no wash steps. Pricing can be found on our PEG SPARCL™ kits webpage.

Rat IgA SPARCL™ Kit – added July 26, 2016
IGA-SP-2

Rat IgM SPARCL™ Kit – added July 21, 2016
IGM-SP-2

Rat IgG SPARCL™ Kit – added July 20, 2016
A Rat IgG SPARCL kit is now available: IGG-SP-2

Mouse IgG2b and IgG3 SPARCL™ Kits – added July 19, 2016
Mouse IgG2b (IGG2B-SP-1) and IgG3 (IGG3-SP-1) SPARCL kits are now available.

Mouse IgG2a SPARCL™ Kit – added July 8, 2016
A Mouse IgG2a SPARCL kit is now available: IGG2A-SP-1

Mouse IgG1 SPARCL™ Kit – added June 30, 2016
A Mouse IgG1 SPARCL kit is now available: IGG1-SP-1

Mouse IgM SPARCL™ Kit – added June 28, 2016
Mouse IgM SPARCL kit: IGM-SP-1

Mouse IgG SPARCL™ Kit – added June 25, 2016
A Mouse IgG SPARCL kit is now available: IGG-SP-1

Monkey IgE SPARCL™ Kit – added June 14, 2016
A SPARCL version of our Monkey IgE ELISA kit is now available: IGE-SP-3

Monkey IgA SPARCL™ Kit – added June 13, 2016
A SPARCL version of our Monkey IgA ELISA kit is now available: IGA-SP-3

Horse SAA SPARCL™ Kit – added June 7, 2016
A SPARCL version of our horse SAA ELISA kit is now available: SAA-SP-14

Monkey IgG and IgM SPARCL™ Kits – added May 27, 2016
Monkey IgG (IGG-SP-3) and IgM (IGM-SP-3) SPARCL kits have been added to our website. They allow measurement of the respective immunoglobulins using simple, 30-minute, no-wash immunoassays.

SPARCL™ Kits – added May 12, 2016
Since November, 2015 the staff at LDI have been busy developing SPARCL™ versions of our ELISA kits. SPARCL™ technology (Spatial Proximity Analyte Reagent Capture) was developed by Lumigen Corp. We are very pleased to announce that Lumigen has granted Life Diagnostics exclusive rights to manufacture SPARCL versions of our ELISA kits. Thus far, we have made 35 kits. They are listed on our SPARCL kits webpage. More are in development.

SPARCL™ assays use two antibodies. One is conjugated to HRP, the second to acridan, a chemiluminescent substrate. When HRP and acridan conjugated antibodies both bind to their target antigen they are brought into close proximity. With the addition of hydrogen peroxide, HRP catalyzes oxidation of proximal acridan molecules causing a flash of chemiluminescence that is proportional to antigen concentration. Acridan conjugated antibodies distant from HRP produce no signal. The technology allows  development of rapid, no-wash, homogeneous immunoassays.

In a typical SPARCL™ assay a mixture of acridan and HRP conjugates is first pipetted into wells of a white 96-well SPARCL™ plate. Diluted samples and standards are then added. After a 30-minute incubation the plate is placed into a luminometer. Luminescence is measured after injection of trigger solution containing hydrogen peroxide, into each well. Assuming 15-minutes preparation and 5-minutes analysis time, the entire assay is complete within 50-minutes. This contrasts with ELISA’s that take 2 – 4+ hours, require 2-4 incubations and 1-3 wash steps.

SPARCL assays require a luminometer capable of simultaneous injection (only one injector is required) and measurement of luminescence. A list of suitable luminometers may be found here.

I have been developing ELISA’s since 2003 and have used them extensively for many years prior to that. I spent the last few months developing our SPARCL™ kits. I am very impressed with their simplicity and speed compared to ELISA.

Christopher Chadwick PhD
President
Life Diagnostics, Inc.

Products in Development – Please inquire regarding availability
– Multiplex assays for animal acute phase proteins, cardiovascular biomarkers and immunoglobulins.