Guinea pig SAA ELISA – added September 15, 2023
Serum amyloid A (SAA) is a positive acute phase protein that can be upregulated as much as 1000-fold in mammalian serum due to inflammation or infection. ELISA kit SAA-7 allows easy measurement of SAA in guinea pig serum. Refer to the Acute Phase Protein ELISA kits page for more information.
Trout IgT ELISA – added September 14, 2023
IgT is a relatively low abundance immunoglobulin that is involved in mucosal immunity in teleost fish. The IgT ELISA kit (IGT-23) allows the measurement of IgT in Rainbow trout serum, plasma, and tissue extracts. Pricing can be found on the Aquaculture Products page.
Cat Paraoxonase ELISA – added August 8, 2023
Paraoxonase 1 (PON1) is considered a useful biomarker of feline infectious peritonitis (FIP). Serum levels decrease in cats with FIP, whereas levels of alpha-1-acid glycoprotein increase (we also manufacture a cat AGP ELISA kit, AGP-8). The PON1 ELISA kit (PON1-8) allows measurement of PON1 in serum (we have not yet tested plasma but expect it to be a suitable test matrix). Pricing of the kit can be found on the Acute Phase Protein ELISA kits page.
Trout LECT2 ELISA – added July 5, 2023
LECT2 (leukocyte cell-derived chemotaxin 2) is a positive acute phase protein. Its levels increase in rainbow trout serum during bacterial infections. The LECT2 ELISA kit (LECT2-23) allows the measurement of LECT2 in Rainbow trout serum and tissue extracts. Pricing can be found on the Aquaculture Products page.
Trout Fibrinogen ELISA – added June 20, 2023
In trout, we found that plasma fibrinogen levels decreased significantly after bacterial challenge. Fibrinogen may therefore provide a useful biomarker for the assessment of fish health. Our fibrinogen ELISA kit (FIB-23) allows easy measurement of fibrinogen in Atlantic Salmon and Rainbow Trout. Pricing can be found on the Aquaculture Products page.
Pig Haptoglobin monoclonal antibody – added March 21, 2023
We have developed a monoclonal antibody for pig haptoglobin (HAPT-9-11D10). Because of the oligomeric nature of haptoglobin, the antibody can be used in ELISA as both coating and conjugated antibody. Please inquire about pricing.
Shrimp HSP40 ELISA – added January 23, 2023
Heat shock protein levels increase in various shrimp tissues during heat stress and bacterial infecttions. The kit (HSP40-24) was developed at Life Diagnostics. Pricing can be found on the Aquaculture Products page.
Trout HSP70 ELISA – added January 17, 2023
Heat shock protein levels increase in tissues including gill, muscle, and liver during heat stress in trout and salmon. The kit (HSP70-23) was developed at Life Diagnostics. Pricing can be found on the Aquaculture Products page.
Trout CFHL-1 ELISA – added January 11, 2023
Complement factor H like 1 (CFHL-1) is involved in regulation of the innate immune response. Levels are high in serum from rainbow trout that are resistant to certain bacterial infections. The kit (CFHL1-23) uses recombinant trout CFHL-1, and antibodies against it that were developed at Life Diagnostics. The kit also recognizes Atlantic salmon CFHL-1. Pricing can be found on the Aquaculture Products page.
Trout GDF-15 ELISA – added December 29, 2022
Growth Differentiation Factor-15 (GDF-15) expression increases during inflammation and sepsis. Elevated levels are associated with appetite suppression. In studies at Life Diagnostics, Inc. we found levels of ~5 ng/ml in plasma from healthy trout. Levels as high as 1000 ng/ml were found in plasma from fish infected with F. psychrophilum and Y. ruckeri. The kit (GDF15-23) uses recombinant trout GDF-15, and antibodies against it that were developed at Life Diagnostics. Pricing can be found on the Aquaculture Products page.
Trout Cathelicidin ELISA – added September 8, 2022
Cathelicidin is an antimicrobial protein that increases in rainbow trout and Atlantic salmon plasma during bacterial infections. Levels in healthy trout are ~1 μg/ml; they can increase to ≥ 100 µg/ml during infection. The kit (CATH-23) uses recombinant cathelicidin, and antibodies developed at Life Diagnostics. Pricing can be found on the Aquaculture Products page.
Trout Vitellogenin ELISA – added August 26, 2022
Vitellogenin is a precursor of egg yolk proteins. It is produced in the liver and transported via the blood to growing oocytes. Its levels increase dramatically in female fish prior to spawning. It has also been reported to behave as a minor acute phase protein and is increased in plasma after exposure to estrogenic pollutants. The kit (VTG-23) uses vitellogenin, and antibodies developed at Life Diagnostics. It can be used for the measurement of vitellogenin in rainbow trout and Atlantic salmon. Pricing can be found on the Aquaculture Products page.
Sea Turtle MRP-126 ELISA – added August 15, 2022
MRP-126 (S100-A12) is a member of the calgranulin protein family. It is an acute phase protein with antibacterial properties. Initial studies indicate that its levels increase significantly in chronically debilitated Loggerhead sea turtles. The ELISA kit (MRP-21) uses recombinant MRP-126, and antibodies developed at Life Diagnostics. Pricing can be found on the acute phase protein ELISA kits page.
Trout Haptoglobin ELISA – added August 5, 2022
Haptoglobin is an acute phase protein that increases in rainbow trout and Atlantic salmon serum during bacterial infections. Levels in healthy fish are undetectable; they can increase to ≥ 8 µg/ml during infection. The kit (HAPT-23) uses recombinant haptoglobin, and antibodies developed at Life Diagnostics. Pricing can be found on the Aquaculture Products page.
Trout C1q-like protein 3 ELISA – added July 28, 2022
C1q-LP3 is an acute phase protein that increases in rainbow trout and Atlantic salmon serum during bacterial infections. Levels in healthy fish are ≤ 0.1 µg/ml; they can increase to ≥ 70 µg/ml during infection. The kit (C1Q-23) uses recombinant C1q-LP3 and antibodies developed at Life Diagnostics. Pricing can be found on the Aquaculture Products page.
Trout and Salmon IgM Antibodies – added June 24, 2022
We have developed two monoclonal antibodies that recognize rainbow trout and Atlantic salmon IgM (IGM-23-2E7, and IGM-23-12B9). Western blots demonstrated that both specifically recognize the IgM heavy chain. They can also be used in immunoassays and flow cytometry. Pricing can be found in the shop (secondary antibody section) and on the Aquaculture Products page.
Trout and Salmon IgM ELISA kit – added June 24, 2022
We now manufacture an ELISA kit for the measurement of rainbow trout and Atlantic salmon IgM (IGM-23). The antibody used in the kit, for both capture and detection, recognizes the IgM heavy chain. The ELISA is a serial assay; 45-min standard/sample incubation; 45 min HRP-conjugate incubation; and a 20-min substrate incubation. Pricing can be found in the shop and on the Aquaculture Products page.
Salmon Skeletal Muscle Troponin-C ELISA kit – added January 24, 2022
Troponin-C is a stable 17 kDa protein that is released from muscle damaged by disease or trauma. The salmon skeletal muscle troponin-C ELISA (STNC-19) is specific for the isoform of troponin-C released fast-twitch skeletal muscle. The kit can measure STNC from Atlantic salmon and rainbow trout (other fish species have not been tested). Pricing can be found in the shop and the Aquaculture Products page.
Salmon Cardiac Troponin-C ELISA kit – added January 24, 2022
Troponin-C is a stable 17 kDa protein that is released from muscle damaged by disease or trauma. The salmon cardiac troponin-C ELISA (CTNC-19) is specific for the isoform of troponin-C released from cardiac muscle and slow-twitch skeletal muscle. The kit can measure CTNC from Atlantic salmon and rainbow trout (other fish species have not been tested). Pricing can be found in the shop and the Aquaculture Products page.
Chicken MRP-126 ELISA kit – added December 16, 2021
MRP-126 is the avian equivalent of the mammalian calgranulin S100A12. It is an acute phase protein that is upregulated in chicken plasma and fecal extracts during bacterial infections. The chicken MRP-126 ELISA kit (MRP-5) was developed at Life Diagnostics using polyclonal antibodies generated against recombinant chicken MRP-126. In fecal extracts, we found levels ranging from 0.01 ug/g in healthy chickens to 14 ug/g in chickens infected with E. maxima. The kit costs $550.
Human and Monkey IgE ELISA kits – added November 12, 2021
Using IgE specific monoclonal antibodies produced at Life Diagnostics, we have developed ELISA kits for human IgE (IGE-20) and monkey IgE (IGE-3-N). Both assays use a co-incubation format and can be completed in less than two hours, including sample preparation time. Kits (96-well) are priced at $500.
Chicken SAA SPARCL™ kit – added August 18, 2021
The chicken serum amyloid A (SAA) SPARCL™ kit (SAA-SP-5) is a 96-well luminescence assay that allows quick and easy measurement of SAA in chicken plasma. Samples (50 μl) and conjugate mixture (25 μl) are co-incubated for 30 minutes in wells of a SPARCL™ microtiter plate. The plate is then inserted into a luminometer and luminescence is measured after injection of trigger solution (37.5 μl/well). A wash-step is not required. SAA concentrations in the samples are derived from a standard curve. SAA is a good inflammatory biomarker in chickens; its levels can increase >50-fold. Pricing information and a list of our other SPARCL™ kits can be found on our SPARCL™ kits page.
Elephant SAA ELISA – added August 17, 2021
At the request of one of our customers, we developed an ELISA kit for elephant serum amyloid A (SAA-22). The assay recognizes Indian elephant SAA. We expect it to recognize African elephant SAA too. SAA is an acute phase protein. Serum levels increase during inflammation and disease. The kit can therefore be used to monitor elephant health. Pricing can be found on our acute phase protein ELISA kits page.
Human Anti-KLH IgG and IgM ELISA kits – added August 17, 2021
KLH is a powerful immunostimulant that is often used as an adjuvant in research studies, and occasionally in clinical applications. The human anti-KLH IgG (KLHG-20) and anti-KLH IgM (KLHM-20) allow quantitive measurement of the respective antibodies in serum, plasma, and ascites fluid. Both kits are priced at $500 each. Other anti-KLH assays are listed on our Immunotoxicity ELISA kits page.
Stabilized Human Cardiac Troponin-I – added July 20, 2021
Stabilized human cardiac troponin-I (1230-R) is a mixture of pure recombinant human cardiac troponin-I and pure recombinant rabbit skeletal muscle troponin-C. Troponin-C forms a complex with troponin-I, rendering the latter soluble and protecting it from proteolytic degradation. We use similar formulations to create standards used in our animal cardiac troponin-I ELISA kits. 1230-R is priced at $450/0.1 mg and $4500/1 mg.
Rabbit Anti-PEG IgG and IgM ELISA kits – added July 9, 2021
Rabbit anti-PEG IgM and IgG ELISA kits (PEGM-10 and PEGG-10) measure levels of anti-PEG IgM and IgG in serum, plasma, and other fluids. Pricing can be found on our PEG ELISA kits page.
Recombinant rabbit skeletal muscle troponin-C – added March 31, 2021
We now manufacture recombinant rabbit skeletal muscle TnC (3011). It is expressed, untagged, in E coli and purified using calcium-dependent affinity chromatography under non-denaturing conditions. It is an excellent alternative to native rabbit skeletal muscle TnC. It can be used to stabilize cardiac troponin-I in immunoassays. It is priced at $150/1 mg and $600/5 mg.
Recombinant human cardiac troponin-T – added March 30, 2021
We now manufacture recombinant human cardiac troponin-T (1111). It is expressed in E coli with an N-terminal his-tag and purified using metal-chelate affinity chromatography. It is offered as an alternative to native human cardiac troponin-T (1110). It is priced at $300/0.1 mg and $1500/mg.
Rabbit CRP ELISA, CRP-10-N – added March 19, 2021
We have developed an improved version of our popular rabbit CRP ELISA kit (CRP-10). The new kit (CRP-10-N) uses rabbit CRP IgY antibodies that were generated by injecting chickens with pure rabbit CRP and affinity purified on rabbit-CRP agarose. The standard used in the kit was calibrated to pure rabbit CRP, the concentration of which was determined by absorbance at 280 nm using the known extinction coefficient. Kit CRP-10 will be discontinued when antibodies used in its production are exhausted. Please refer to the kit instructions for more details.
Human Anti-PEG IgM ELISA – added February 15, 2021
The human anti-PEG IgM ELISA (PEGM-20) measures levels of anti-PEG IgM in serum, plasma, and other fluids. In studies at Life Diagnostics, we found that significantly more samples had measurable anti-PEG IgM than had measurable anti-PEG IgG. The kit is strictly for research use. It cannot be used for diagnostic purposes. Pricing can be found on our PEG ELISA kits page.
Human Anti-PEG IgG ELISA – added February 11, 2021
Over the past few months, we have observed significantly increased interest in human anti-PEG antibodies. We have therefore developed a human anti-PEG IgG ELISA kit (PEGG-20). The kit is strictly for research use only. It cannot be used for diagnostic purposes. Pricing can be found on our PEG ELISA kits page.
Horse SAA monoclonal antibodies – added February 4, 2021
We have developed three monoclonal antibodies for horse SAA, an acute phase protein that is an excellent biomarker for inflammation and tissue injury. Serum levels can increase up to 1000-fold. We offer three monoclonals; SAA-14-10D5 can be used in ELISA as an HRP conjugate, paired SAA-14-9B1 or SAA-14-16C10 as capture antibody. Please contact us for prices. Refer to the acute phase protein antibody page for a list of our other antibodies.
Cow SAA3 monoclonal antibodies – added December 31, 2020
Working with Veterinary Biomarkers, Inc., we have developed antibodies for cow SAA3, the isoform of serum amyloid A expressed in milk. SAA3 is an acute phase protein. Levels increase in milk during infections. It is a potential biomarker for mastitis. Currently, we offer two monoclonals; SAA3-11-5H6 and SAA3-11-11H3, that can be paired in immunoassays. Please contact us for prices.
Recombinant acute phase proteins and inflammatory biomarkers – added December 30, 2020
We now manufacture the recombinant acute phase proteins and inflammatory biomarkers listed below. ELISA kits are available for chicken EX-FABP (EXFABP-5), dog NGAL (NGAL-4), and dog PAP1 (PAP1-4). ELISA kits for others will follow soon.
|Dog PAP1 (pancreatitis associated protein-1)||PAP1-4-0.05|
|Cat PAP1 (pancreatitis associated protein-1)||PAP1-8-0.05|
Prices are listed on the acute phase proteins page.
Dog Pancreatitis Associated Protein-1 (PAP-1) ELISA – added July 21, 2020
PAP-1 is an acute phase protein. Its expression is very low in healthy pancreatic tissue but increases significantly during acute pancreatitis and other diseases. In studies at LDI, we found that fecal levels of PAP-1 increased significantly in dogs with IBD. The dog PAP-1 ELISA (PAP1-4) uses dog PAP-1 specific antibodies developed at LDI and is calibrated to pure dog PAP-1. It can be used for the measurement of PAP-1 in fecal extracts, serum, and plasma (other fluids have not yet been tested). A single 96-well kit is priced at $550. Volume discounts apply.
Human SAA ELISA – added April 8, 2020
Serum amyloid A has recently been identified as a potential biomarker for severe Covid-19 patients. We are pleased to launch our human SAA ELISA (SAA-20), available for research use only. Refer to our acute phase protein ELISA kits page for pricing.
Anti-Cow Haptoglobin Monoclonal Antibody – added March 19, 2020
We have recently added another cow haptoglobin monoclonal to our catalog. HAPT-11-6A8 pairs with HAPT-11-3F7 in SPARCL™ assays. Its performance in ELISA is being investigated. Call or email for pricing.
Horse CRP – added February 17, 2020
We now manufacture horse CRP (8108). CRP is an acute phase protein. Its levels increase during bacterial infections and inflmmatory conditions. It is available as 0.1 mg ($320) and 1 mg ($1600) quantities.
Cow SAA3 ELISA – added December 31, 2019
SAA3 is the isoform of serum amyloid A that is present in milk. It is sometimes referred to as milk amyoid A (MAA). Levels increase because of inflammation and infection associated with mastitis. As shown in the graph below, we find that SAA3 levels correlate with somatic cell count (SCC). Increased SCC correlate with decreased milk quality. The cow SAA3 ELISA (SAA3-11) was developed by Veterinary Biomarkers, Inc. It is simpler, faster, more sensitive (5x), and less expensive than other SAA3 ELISA kits. A single kit costs $450. Bulk discounts apply.