Chicken SAA SPARCL™ kit – added August 18, 2021
The chicken serum amyloid A (SAA) SPARCL™ kit (SAA-SP-5) is a 96-well luminescence assay that allows quick and easy measurement of SAA in chicken plasma. Samples (50 μl) and conjugate mixture (25 μl) are co-incubated for 30 minutes in wells of a SPARCL™ microtiter plate. The plate is then inserted into a luminometer and luminescence is measured after injection of trigger solution (37.5 μl/well). A wash-step is not required. SAA concentrations in the samples are derived from a standard curve. SAA is a good inflammatory biomarker in chickens; its levels can increase >50-fold. Pricing information and a list of our other SPARCL™ kits can be found on our SPARCL™ kits page.
Elephant SAA ELISA – added August 17, 2021
At the request of one of our customers, we developed an ELISA kit for elephant serum amyloid A (SAA-22). The assay recognizes Indian elephant SAA. We expect it to recognize African elephant SAA too. SAA is an acute phase protein. Serum levels increase during inflammation and disease. The kit can therefore be used to monitor elephant health. Pricing can be found on our acute phase protein ELISA kits page.
Human Anti-KLH IgG and IgM ELISA kits – added August 17, 2021
KLH is a powerful immunostimulant that is often used as an adjuvant in research studies, and occasionally in clinical applications. The human anti-KLH IgG (KLHG-20) and anti-KLH IgM (KLHM-20) allow quantitive measurement of the respective antibodies in serum, plasma, and ascites fluid. Both kits are priced at $500 each. Other anti-KLH assays are listed on our Immunotoxicity ELISA kits page.
Stabilized Human Cardiac Troponin-I – added July 20, 2021
Stabilized human cardiac troponin-I (1230-R) is a mixture of pure recombinant human cardiac troponin-I and pure recombinant rabbit skeletal muscle troponin-C. Troponin-C forms a complex with troponin-I, rendering the latter soluble and protecting it from proteolytic degradation. We use similar formulations to create standards used in our animal cardiac troponin-I ELISA kits. 1230-R is priced at $450/0.1 mg and $4500/1 mg.
Rabbit Anti-PEG IgG and IgM ELISA kits – added July 9, 2021
Rabbit anti-PEG IgM and IgG ELISA kits (PEGM-10 and PEGG-10) measure levels of anti-PEG IgM and IgG in serum, plasma, and other fluids. Pricing can be found on our PEG ELISA kits page.
Recombinant rabbit skeletal muscle troponin-C – added March 31, 2021
We now manufacture recombinant rabbit skeletal muscle TnC (3011). It is expressed, untagged, in E coli and purified using calcium-dependent affinity chromatography under non-denaturing conditions. It is an excellent alternative to native rabbit skeletal muscle TnC. It can be used to stabilize cardiac troponin-I in immunoassays. It is priced at $150/1 mg and $600/5 mg.
Recombinant human cardiac troponin-T – added March 30, 2021
We now manufacture recombinant human cardiac troponin-T (1111). It is expressed in E coli with an N-terminal his-tag and purified using metal-chelate affinity chromatography. It is offered as an alternative to native human cardiac troponin-T (1110). It is priced at $300/0.1 mg and $1500/mg.
Rabbit CRP ELISA, CRP-10-N – added March 19, 2021
We have developed an improved version of our popular rabbit CRP ELISA kit (CRP-10). The new kit (CRP-10-N) uses rabbit CRP IgY antibodies that were generated by injecting chickens with pure rabbit CRP and affinity purified on rabbit-CRP agarose. The standard used in the kit was calibrated to pure rabbit CRP, the concentration of which was determined by absorbance at 280 nm using the known extinction coefficient. Kit CRP-10 will be discontinued when antibodies used in its production are exhausted. Please refer to the kit instructions for more details.
Human Anti-PEG IgM ELISA – added February 15, 2021
The human anti-PEG IgM ELISA (PEGM-20) measures levels of anti-PEG IgM in serum, plasma, and other fluids. In studies at Life Diagnostics, we found that significantly more samples had measurable anti-PEG IgM than had measurable anti-PEG IgG. The kit is strictly for research use. It cannot be used for diagnostic purposes. Pricing can be found on our PEG ELISA kits page.
Human Anti-PEG IgG ELISA – added February 11, 2021
Over the past few months, we have observed significantly increased interest in human anti-PEG antibodies. We have therefore developed a human anti-PEG IgG ELISA kit (PEGG-20). The kit is strictly for research use only. It cannot be used for diagnostic purposes. Pricing can be found on our PEG ELISA kits page.
Horse SAA monoclonal antibodies – added February 4, 2021
We have developed three monoclonal antibodies for horse SAA, an acute phase protein that is an excellent biomarker for inflammation and tissue injury. Serum levels can increase up to 1000-fold. We offer three monoclonals; SAA-14-10D5 can be used in ELISA as an HRP conjugate, paired SAA-14-9B1 or SAA-14-16C10 as capture antibody. Please contact us for prices. Refer to the acute phase protein antibody page for a list of our other antibodies.
Cow SAA3 monoclonal antibodies – added December 31, 2020
Working with Veterinary Biomarkers, Inc., we have developed antibodies for cow SAA3, the isoform of serum amyloid A expressed in milk. SAA3 is an acute phase protein. Levels increase in milk during infections. It is a potential biomarker for mastitis. Currently, we offer two monoclonals; SAA3-11-5H6 and SAA3-11-11H3, that can be paired in immunoassays. Please contact us for prices.
Recombinant acute phase proteins and inflammatory biomarkers – added December 30, 2020
We now manufacture the recombinant acute phase proteins and inflammatory biomarkers listed below. ELISA kits are available for chicken EX-FABP (EXFABP-5), dog NGAL (NGAL-4), and dog PAP1 (PAP1-4). ELISA kits for others will follow soon.
|Dog PAP1 (pancreatitis associated protein-1)||PAP1-4-0.05|
|Cat PAP1 (pancreatitis associated protein-1)||PAP1-8-0.05|
Prices are listed on the acute phase proteins page.
Dog Pancreatitis Associated Protein-1 (PAP-1) ELISA – added July 21, 2020
PAP-1 is an acute phase protein. Its expression is very low in healthy pancreatic tissue but increases significantly during acute pancreatitis and other diseases. In studies at LDI, we found that fecal levels of PAP-1 increased significantly in dogs with IBD. The dog PAP-1 ELISA (PAP1-4) uses dog PAP-1 specific antibodies developed at LDI and is calibrated to pure dog PAP-1. It can be used for the measurement of PAP-1 in fecal extracts, serum, and plasma (other fluids have not yet been tested). A single 96-well kit is priced at $550. Volume discounts apply.
Human SAA ELISA – added April 8, 2020
Serum amyloid A has recently been identified as a potential biomarker for severe Covid-19 patients. We are pleased to launch our human SAA ELISA (SAA-20), available for research use only. Refer to our acute phase protein ELISA kits page for pricing.
Anti-Cow Haptoglobin Monoclonal Antibody – added March 19, 2020
We have recently added another cow haptoglobin monoclonal to our catalog. HAPT-11-6A8 pairs with HAPT-11-3F7 in SPARCL™ assays. Its performance in ELISA is being investigated. Call or email for pricing.
Horse CRP – added February 17, 2020
We now manufacture horse CRP (8108). CRP is an acute phase protein. Its levels increase during bacterial infections and inflmmatory conditions. It is available as 0.1 mg ($320) and 1 mg ($1600) quantities.
Cow SAA3 ELISA – added December 31, 2019
SAA3 is the isoform of serum amyloid A that is present in milk. It is sometimes referred to as milk amyoid A (MAA). Levels increase because of inflammation and infection associated with mastitis. As shown in the graph below, we find that SAA3 levels correlate with somatic cell count (SCC). Increased SCC correlate with decreased milk quality. The cow SAA3 ELISA (SAA3-11) was developed by Veterinary Biomarkers, Inc. It is simpler, faster, more sensitive (5x), and less expensive than other SAA3 ELISA kits. A single kit costs $450. Bulk discounts apply.