Alpha-1-Acid glycoprotein (A1AGP) is one if the most acidic proteins found in serum. It has a molecular weight of approximately 44 kDa with a high (~45%) content of carbohydrate. The molecular weight of the non-glycosylated polypeptide is 23.5 kDa. It is a positive acute phase reactant, the levels of which increase significantly in rats in response to inflammation, tissue injury and disease. A1AGP is purified using a combination of acid extraction, precipitation, gel filtration and ion-exchange chromatography steps.