PEG and Anti-PEG ELISA Kits
We manufacture ELISA kits for measurement of methoxy-PEG and non-methoxy-PEG (free and conjugated) as well as ELISA kits for detection of anti-PEG IgM and IgG in mouse, rat, monkey, and human serum or plasma.
PEG coated plates and blocking buffers are available for those interested in developing their own PEG assays. Researchers should be aware that many commercially available ELISA buffers contain Tween-20 or other polyoxyethylene detergents that significantly interfere with PEG assays. Our blocking buffer is devoid of polyoxyethylene detergents and can be used to block and stabilize plates coated with PEGylated proteins or PEG antibodies.
In December 2024, we began updating our ELISA kits for measurement of anti-PEG IgG and IgM. The kits remain the same, except that the HRP-conjugate is provided as a stable lyophilized stock that must be reconstituted and diluted prior to use. The new kits are identified with a “-N” suffix.
Orders may be placed by phone, fax, email, or via our online shop.
|
ELISA Kit / Reagent |
Instructions / Information sheet |
Cat. # |
Price |
| PEG ELISA – Competitive ELISA for detection of PEGylated proteins | Insert | PEG | $575 |
| PEG ELISA – Sandwich ELISA for detection of PEGylated proteins | Insert | PEG-N | $600 |
| mPEG ELISA – Sandwich ELISA for detection of mPEGylated proteins | Insert | MPEG | $600 |
| Mouse Anti-PEG IgG ELISA | Insert | PEGG-1-N | $650 |
| Mouse Anti-PEG IgM ELISA |
Insert | PEGM-1-N | $650 |
| Rat Anti-PEG IgG ELISA |
Insert | PEGG-2-N | $650 |
| Rat Anti-PEG IgM ELISA |
Insert | PEGM-2-N | $650 |
| Monkey Anti-PEG IgG ELISA |
Insert | PEGG-3-N | $650 |
| Monkey Anti-PEG IgM ELISA |
Insert | PEGM-3-N | $650 |
| Rabbit Anti-PEG IgG ELISA |
Insert | PEGG-10-N | $650 |
| Rabbit Anti-PEG IgM ELISA |
Insert | PEGM-10-N | $650 |
| Human Anti-PEG IgG ELISA |
Insert | PEGG-20-N | $650 |
| Human Anti-PEG IgM ELISA |
Insert | PEGM-20-N | $650 |
| PEG-BSA Coated plate – Coated with mono mPEG-BSA (20 kDa) |
Info | PBSA20PL | $325 |
| PEG Blocker, 125 ml | Info | PEGB125-1 | $125 |
| PEG Diluent, 50 ml – (Not for MPEG kit) | Info | PEGD50-1 | $50 |
| PEG Wash, 50 ml (20x) – (Not for MPEG kit) | Info | PEGW50-20 | $50 |
For technical advice please email us at [email protected].
The figures below illustrate the use of our mouse anti-PEG IgG and IgM ELISA kits. Mice were immunized with mPEG-KLH (n = 5 for each study). Serum samples collected on days 7 and 25 were used for evaluation of anti-PEG IgM and IgG respectively. Antibody levels were evaluated using microtiter plates coated with (i) methoxy-PEG-BSA (PBSA-01) and (ii) a comparable non-methoxy PEG-BSA, in which the terminal methoxy group was replaced by a pentanoic acid group (PBSA-01P). Comparison of reactivity on the two plates allowed assessment of antibodies directed against the terminal methoxy group as well as the PEG backbone. As can be seen, in the IgM study (upper figure), serum from all five mice gave identical reactivities against methoxy and non-methoxy PEG-BSA, indicating that all mice produced IgM antibodies directed against the PEG backbone. In the IgG study (lower figure), four of the five mice gave identical reactivities against both PEG antigens, again indicating that all antibodies produced by those mice were directed against the PEG-backbone. However, one mouse (M888) had a 3-fold greater reactivity against methoxy-PEG BSA, indicating a predominant antibody response against the terminal methoxy group.
Please note that our anti-PEG ELISA kits (listed above) are provided with methoxy-PEG coated plates. non-methoxy PEG coated plates are available on request.
References
- Im H-J, et al. Accelerated blood clearance phenomenon reduces the passive targeting of PEGylated nanoparticles in peripheral arterial disease. Applied Materials and Interfaces. 8:17955-17963 (2016)