Cardiac Biomarker ELISA Kits

Life Diagnostics Inc., manufactures ELISA kits for cardiac troponin-I, myoglobin, fatty acid binding protein, and myosin light chain-1. Kits are available for species including mouse, rat, rabbit, dog, pig, and monkey. Our kits are widely referenced in the scientific literature.

IMPORTANT: Serum or plasma that is to be used for measurement of cardiac biomarkers must not be collected by cardiac puncture or any other method that causes damage to the heart. Cardiac puncture releases very high levels of cardiac biomarkers into the blood thereby invalidating study results.

Literature references detailing the use of our cardiac biomarker ELISA kits are listed at the bottom of this page.

Orders may be placed by phone, fax, email, or via our online shop.

For technical advice please email us at [email protected]. All kits are manufactured by Life Diagnostics, Inc.

The figure below illustrates the utility of our animal troponin-I, myoglobin, and FABP kits. It shows the levels of the respective markers in plasma obtained from a rabbit cardiac ischemia/reperfusion model. Plasma samples were collected before/during ischemia and at various times after reperfusion. Consistent with clinical findings in humans, it can be seen that  (i) the levels of myoglobin and FABP increase prior to those of troponin-I and (ii) troponin-I levels remain elevated longer than do those of myoglobin and FABP.

Mouse cTnI Publications

1. Lackner I, et al. Toll-like receptor-mediated cardiac injury during experimental sepsis. Mediators of Inflammation. Volume 2020, Article ID 6051983
2. Luan HL, et al. GDF15 is an inflammation-induced central mediator of tissue tolerance. Cell. 178:1231-1244 (2019).
3. Grose JH, et al. Characterization of the cardiac overexpression of HSPB2 reveals mitochondrial and myogenic roles supported by a cardiac HspB2 Interactome. (2013). https://doi.org/10.1371/journal.pone.0133994
4. Tajiri K, et al. Pitavastatin regulates helper T-cell differentiation and ameliorates autoimmune myocarditis in mice. Cardiovasc Drugs Ther. [Epub ahead of print] (2013)
5. Antoniak S, et al. PAR-1 contributes to the innate immune response during viral infection. J Clin Invest. [Epub ahead of print] (2013) http://www.jci.org/articles/view/66125
6. Butcher A, et al. An allosteric modulator of the adenosine A1 receptor improves cardiac function following ischaemia in murine isolated hearts. Pharmaceuticals. 6(4):546-556 (2013) http://www.mdpi.com/1424-8247/6/4/546
7. Taghiabadi E, et al. Protective effect of silymarin against acrolein-induced cardiotoxicity in mice. Evid Based Complement Alternat Med. 352091 (2012) http://www.hindawi.com/journals/ecam/2012/352091/
8. Foy KC, et al. Immunotherapy with HER-2 and VEGF peptide mimics plus metronomic paclitaxel causes superior antineoplastic effects in transplantable and transgenic mouse models of human breast cancer. OncoImmunology. 1(7):1-13 (2012) http://www.landesbioscience.com/journals/53/article/21057/
9. Zhang Y, et al. The comparison of α-bromo-4-chlorocinnamaldehyde and cinnamaldehyde on coxsackie virus B3-induced myocarditis and their mechanisms. Int Immunopharmacol. 14(1):107-113 (2012)
10. Bhushan S, et al. Selective β2-Adrenoreceptor Stimulation Attenuates Myocardial Cell Death and Preserves Cardiac Function After Ischemia-Reperfusion Injury. Arterioscler Thromb Vasc Biol. 32(8):1865-1874 (2012) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401356/
11. Predmore BL, et al. The polysulfide diallyl trisulfide protects the ischemic myocardium by preservation of endogenous hydrogen sulfide and increasing nitric oxide bioavailability. Am J Physiol Heart Circ Physiol. 302(11):H2410-8 (2012)
12. De Meyer SF, et al. Protective anti-inflammatory effect of ADAMTS13 on myocardial ischemia/reperfusion injury in mice. Blood. [Epub ahead of print] (2012)
13. Peart JN, See Hoe L, Pepe S, Johnson P and Headrick JP. Opposing effects of age and calorie restriction on molecular determinants of myocardial ischemic tolerance. Rejuvenation Res. 15(1):59-70 (2012)
14. Kean TJ, et al. Development of a peptide-targeted, myocardial ischemia-homing, mesenchymal stem cell. J Drug Target. 20(1): 23-32 (2012)
15. Xu Z, Desai M, Philip J, Sivsubramanian N, Bowles NE and Vallejo JG. Conditional transgenic expression of TIR-domain-containing adaptor-inducing interferon-β (TRIF) in the adult mouse heart is protective in acute viral myocarditis. Basic Res Cardiol. 106(6):1159-1171 (2011)
16. Kiraly A, Koffman B, Hacker M, Gunning W, Rasche S, Quinn A. A novel aza-anthrapyrazole blocks the progression of experimental autoimmune encephalomyelitis after the priming of autoimmunity. Clin Immunol. 141(3):304-316 (2011)
17. Ling Y, Pong T, Vassiliou CC, Huang PL and Cima MJ. Implantable magnetic relaxation sensors measure cumulative exposure to cardiac biomarkers. Nat Biotechnol. 29(3):273-277 (2011)
18. Xu B, Strom J and Chen QM. Dexamethasone induces transcriptional activation of Bcl-xL gene and inhibits cardiac injury by myocardial ischemia. Eur J Pharmacol. 668(1-2):194-200 (2011)
19. Braunersreuther V, et al. Chemokine CCL5/RANTES inhibition reduces myocardial reperfusion injury in atherosclerotic mice. J Mol Cell Cardiol. 48(4):789-798 (2010)
20. Haas MS, Alicot EM, Schuerpf F, Chiu I, Li J, Moore FD and Carrol MC. Blockade of self-reactive IgM significantly reduces injury in a murine model of acute myocardial infarction. Cardiovascular Research 87:618-627 (2010)
21. Maas JE, Wan TC, Figler RA, Gross GJ and Auchampach JA. Evidence that the acute phase of ischemic preconditioning does not require signaling by the A 2B adenosine receptor. J Mole Cell Cardiol. 49(5):886-893 (2010) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2958103/
22. Cao Z, Hu Y, Wu W, Ha T, Kelley J, Deng C, Chen Q, Li J and Li Y. The TIR/BB-loop mimetic AS-1 protects the myocardium from ischemia/reperfusion injury. Cardiovascular Research 84:442-451 (2009)
23. Engle SK, et al. Qualification of cardiac troponin-I concentration in mouse serum using isoproterenol and implementation in pharmacology studies to accelerate drug development. Toxicologic Pathology 37:617-628 (2009)
24. Qi D, Hu X. Wu X, Merk M, Leng L, Bucala R and Young LH. Cardiac Macrophage migration inhibitory factor inhibits JNK pathway activation and injury during ischemia/reperfusion. J Clin Invest. 119:3807-3816 (2009)
25. Richer MJ, Lavallée DJ, Shanina I, Horwitz MS.  Toll-Like Receptor 3 Signaling on Macrophages Is Required for Survival Following Coxsackievirus B4 Infection. PLoS ONE 4(1): e4127. doi:10.1371/journal.pone.0004127 (2009)
26. Atkinson C., et al. Complement-dependent inflammation and injury in a murine model of brain dead donor hearts. Circulation Research. 105:1094-1101 (2009)
27. Buyse MG, et al. Long-term blinded placebo-controlled study of SNT-MC17/idebenone in the dystrophin deficient mdx mouse: cardiac protection and improved exercise performance. Eurpoean Heart Journal 30:116-124 (2009)
28. Elrod JW et.al. Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function. PNAS 104:15560-15565 (2007)
29. Eckle T, et al. Cardioprotection by Ecto-5′-Nucleotidase (CD73) and A2B Adenosine Receptors. Circulation 115:1581-1590 (2007)
30. Eckle T, et al. Systematic evaluation of a novel model for cardiac ischemic preconditioning in mice. Am J Physiol Heart Circ. Physiol. 291:H2533-H2540 (2006)
31. Hardarson HS, et al. Toll-like receptor 3 is an essential component of the innate stress response in virus-induced cardiac injury. Am J Physiol Heart Circ. Physiol 292:H251-H258 (2006)
32. Madonna R, Rinaldi L, Rossi C, Geng Y-J and De Caterina R. Prostacyclin improves transcoronary myocardial delivery of adipose tissue-derived stromal cells. Clinical Research 27:2054-2061 (2006)
33. Morrison RR, Tan XL, Ledent C, Mustafa SJ and Hifmann PA. Targeted deletion of A2A adenosine receptors attenuates the protective effects of myocardial postconditioning. Am J Physiol Heart Circ Physiol. 293:H2523-H2529 (2007)
34. Engle, SK et al.  Strain differences in serum cardiac troponin-I concentration and myocardial necrosis after isoproterenol treatment in mice.  Abstract No. 383.  The Toxicologist CD — An official Journal of the Society of Toxicology, Volume 96, Number S-1 (2007)
35. Hou G, Dick R, Zeng C, Brewer GJ. Comparison of lowering copper levels with tetrathiomolybdate and zinc on mouse tumor and doxorubicin models. Translational Research 148:309-314 (2006)
36. Huber SA, Feldman AM and Sartini D. Coxsackievirus B3 induces T regulatory cells, which inhibit cardiomyopathy in tumor necrosis factor-a transgenic mice. Circ Res. 99:1109-1116 (2006)
37. Mullick A, Leon Z, Min-Oo G, Berghout J, Lo R, Daniels E and Gros P. Cardiac failure in C5-deficient mice after Candida albicans infection. Infection and Immunity. 74(8):4439-4451 (2006)Hou G, Dick R, Abrams GD, Brewer GJ., Tetrathiomolybdate protects against cardiac damage by doxorubicin in mice. J Lab Clin Med.146(5):299-303 (2005Tao L, Liu H-R, Gao F, Qu Y, Christopher TA, Lopez BL and Ma XL. Mechanical traumatic injury without circulatory shock causes cardiomyocyte apoptosis: rol of reactive nitrogen and reactive oxygen species. Am J Heart Circ Physiol.  288:H2811-H2818 (2005)

Rat cTnI Publications

1. Funcke S, et al. Effects of the delta opiod receptor agonist DADLE in a novel hypoxia-reoxygenation model on human and rat-engineered heart tissue: A pilot study. Biomolecules 10(9):1309 (2020)
2. Yeh CH, et al. Potent cardioprotection from ischemia-reperfusion injury by a 2-domain fusion protein comprising Annexin V and Kunitz protease inhibitor. J Thromb Haemost. doi: 10.1111/jth.12314 (2013)
3. Doods H and Wu D. Sabiporide reduces ischemia-induced arrhythmias and myocardial infarction and attenuates ERK phosphorylation and iNOS induction in rats. BioMed Res Int. 2013:504320 (2013) http://www.hindawi.com/journals/bmri/2013/504320/
4. Ke D, Fang J, Fan L, Chen Z, Chen L. Regulatory T cells contribute to rosuvastatin-induced cardioprotection against ischemia-reperfusion injury. Coron Artery Dis. [Epub ahead of print] (2013)
5. Watts JA, Gellar MA, Fulkerson MB, Kline JA. A soluble guanylate cyclase stimulator, BAY 41-8543, preserves right ventricular function in experimental pulmonary embolism. Pulm Pharmacol Ther. [Epub ahead of print] (2012)
6. Cheng K, et al. Magnetic enhancement of cell retention, engraftment and functional benefit after intracoronary delivery of cardiac-derived stem cells in a rat model of ischemia/reperfusion. Cell Transplant. 21(6):1121-1135 (2012) http://www.ingentaconnect.com/content/cog/ct/2012/00000021/00000006/art00006
7. Juneman EB, Saleh L, Lancaster JJ, Thai HM, Markham B and Goldman S. The effects of poloxamer-188 on left ventricular function in chronic heart failure after myocardial infarction. J Cardiovasc Pharmacol. 60(3):293-298 (2012)
8. Govindan S, et al. Cardiac myosin binding protein-C is a potential diagnostic biomarker for myocardial infarction. J Mol Cell Cardiol. 52(1):154-164 (2012)
9. Huang CH, et al. Acute cardiac dysfunction after short-term diesel exhaust particles exposure. Toxicol Lett. 192(3):349-355 (2012)
10. Kitazume S, et al. Soluble amyloid precursor protein 770 is released from inflamed endothelial cells and activated platelets: a novel biomarker for acute coranory syndrome. J Biol Chem. 287:40817-40825 (2012)
11. Shin IW, et al. Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model. Korean J Anesthesiol. 61(6):499-505 (2011) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249573/
12. Li T, et al. Polymerized human placenta hemoglobin given before ischemia protects rat heart from ischemia reperfusion injury. Artif Cells Blood Substit Immobil Biotechnol. 39(6):392-397 (2011)
13. Zisa D, et al. Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair. Am J Physiol Heart Circ Physiol. 301(6):H2422-2432 (2011)
14. Shin IW, et al. Propofol has delayed myocardial protective effects after a regional ischemia/reperfusion injury in an in vivo rat heart model. Korean J Anesthesiol. 58(4):378-382 (2010) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876860/
15. Todorova VK, Kaufman Y, Hennings LJ and Klimberg VS. Glutamine regulation of doxorubicin accumulation in hearts versus tumors in experimental rats. Cancer Chemother Pharmacol 66:315-323 (2010)
16. Ji X, Takahashi R, Hiura Y, Hirokawa G, Fukushima Y and Iwai N. Plasma miR-208 as a Biomarker of Myocardial Injury. Clin Chem. 55(11):1944-1949 (2009)
17. Yue T-L, et.al. In vivo activation of peroxisome proliferator-activated redeptor-d protects the heart from ischemia/reperfusion injury in Zucker fatty rats. J. Pharm. Exp. Ther. 325:466-474 (2008)
18. Zagorski J, Gellar MA, Obraztsova M, Kline JA and Watts JA. Inhibition of CINC-1 decreases right ventricular damage caused by experimental pulmonary embolism in rats. The Journal of Immunology 179:7820-7826 (2007)
19. Legare JF, Oxner A, Heimrath O, Myers T and Currie RW. Heat Shock treatment results in increased recruitment of labeled PMN following myocardial infarction. Am J Physiol Heart Circ Physiol. 293(5):H3210-5 (2007)
20. Chopra M and Sharma AC. Distinct cardiodynamic and molecular chracteristics during early and late stages of sepsis-induced myocardial dysfunction. Life Sciences. epub. July 2007
21. Liu J, Pang Y, Chang T, Bounelis P, Chatham JC, and Marchase RB. Increased hexoseamine biosynthesis and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia. J Mol. Cell Cardiol. 40 303-312 (2006)
22. Wang P and Chatham JC. Onset of diabetes in Zucker diabetic fatty (ZDF) rats leads to improved recovery of function after ischemia in the isolated perfused heart. Am J Physiol Endocrinol Metab 286:E725-E736 (2004)

Rabbit cTnI Publications

1. Booth EA, and Lucchesi BR. Medroxyprogesterone acetate prevents the cardioprotective and anti-inflammatory effects of 17beta-estradiol in an in vivo model of myocardial ischemia and reperfusion. Am J Physiol Heart Circ Physiol. 293(3):H1408-15 (2007)
2. Lauver DA, Booth EA, White AJ, Poradosu E & Lucchesi BR. Sulodexide attenuates myocardial ischemia/reperfusion injury and the deposition of C-reactive protein in areas of infarction without affecting hemostasis. J Pharm. Exp. Ther. 312(2):794-800 (2005)
3. Lauver DA, Lockwood SF and Lucchesi BR. Disodium disuccinate astaxanthin (Cardax) attenuates complement activation and reduces myocardial injury following ischemia/reperfusion. J Pharm. Exp. Ther. 314(2):686-692 (2005)
4. Booth EA, Obeid NR and Lucchesi BR. Activation of estrogen receptor-alpha protects the in vivo rabbit heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 289(5):H2039-47 (2005)

Pig cTnI Publications

1. Weber B, et al. Systemic and cardiac alterations after long bone fracture. Shock. 54(6):761-773 (2020)
2. Weil BR, et al. Brief myocardial ischemia produces cardiac troponin I release and focal myocyte apoptosis in the absence of pathological infarction in swine. J Am Coll Cardiol Basic Trans Science. 2017;2(2):105-14
3. Uitterdijk A, et al. Serial measurement of hFABP and high sensitivity Troponin I post PCI in STEMI. How fast and accurate can Myocardial Infarct Size and No-Reflow be predicted? Am J Physiol Heart Circ Physiol. [Epub ahead of print] (2013)
4. Wu D, Kraut J, and Abraham WM. Sabiporide improves cardiovascular function, decreases the inflammatory response and reduces mortality in acute metabolic acidosis in pigs. PLoS one. 8(1): e53932 (2013) http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0053932
5. Wu D and Qi J. Mechanisms of the beneficial effect of NHE1 inhibitor in traumatic hemorrhage: inhibition of inflammatory pathways. Resuscitation. 83(6):774-781 (2012)
6. Gill RS, et al. Cyclosporine treatment improves cardiac function and systemic hemodynamics during resuscitation in a newborn piglet model of asphyxia: a dose-response study. Crit Care Med. 40(4): 1237-1244 (2012)
7. Bebarta VS, et al. Hydroxocobalamin and Epinephrine Both Improve Survival in a Swine Model of Cyanide-Induced Cardiac Arrest. Ann Emerg Med. [Epub ahead of print] (2012)
8. Wu D, Qi J, Dai H, Doods H and Abraham WM. Resuscitation with Na+/H+ exchanger inhibitor in traumatic haemorrhagic shock: cardiopulmonary performance, oxygen transport and tissue inflammation. Clin Exp Pharmacol Physiol. 37(3):337-342. (2010)
9. Liu J-Q, Lee T-F, Bigam DL and Cheung P-Y. Effects of post-resuscitation treatment with N-acetylcysteine on cardiac recovery in hypoxic newborn piglets. PLoS one 5(12): e15322 (2010)
10. Osipov RM, et al. Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass. Interactive CardioVascular and Thoracic Surgery 10: 506-512 (2010)
11. Adams JA, et al. Periodic acceleration (pGz) prior to whole body ischemia reperfusion injury provides early cardioprotective preconditioning. Life Sciences 86: 707-715 (2010)
12. Wu D, Bassuk J, Arias J, Doods H and Adams JA. Cardiovascular effects of Na+/H+ exchanger inhibition with BIIB513 following hypovolemic circulatory shock. Shock 23(3):269-274 (2005)

Monkey cTnI Publications

1. Minimo H, et al. Characteristics of troponins as myocardial damage biomarkers in cynomolgus monkeys. J Toxicol Sci. 34(6): 589-601 (2009)

Mouse Myoglobin Publications

1. Nogales-Gadea G, et al. Knock-in mice for the R50X mutation in the PYGM gene present with McArdle disease. Brain. 135(Pt 7): 2048-2057 (2012)
2. Ling Y, Pong T, Vassiliou CC, Huang PL, Cima MJ. Implantable magnetic relaxation sensors measure cumulative exposure to cardiac biomarkers. Nat Biotechnol. 29(3):273-277 (2011)

Rat Myoglobin Publications

1. Ohta M, Kawano H, Notsu T, Naba H and Imada K. Eicosapentaenoic acid attenuates statin-induced ER stress and toxicity in myoblast. Biochem Biophys Res Commun. 424(2):301-307 (2012)
2. Kerkweg U, Pamp K, Fieker J, Petrat F, Hider RC and de Groot H. Release of redox-active iron by muscle crush trauma: no liberation into the circulation. Shock. 33(5):513-518 (2010) http://journals.lww.com/shockjournal/Fulltext/2010/05000/Release_of_Redox_Active_Iron_By_Muscle_Crush.11.aspx

Rat Cardiac FABP Publications

1. Hasic S, et al. Heart-type fatty acid-binding protein and its relation with morphological changes in rat myocardial damage model induced by isoproterenol. Bosn J Basic Med Sci. 11(4):240-244 (2011) http://bjbms.org/archives/2011/4/240