Immunotoxicity ELISA Kits
Anti-KLH, anti-SRBC, anti-tetanus toxoid, anti-DNP and anti-TNP IgM and IgG are used as biomarkers of immunotoxicity. Life Diagnostics manufactures ELISA kits for these biomarkers in mice, rats, chickens, monkeys, and humans.
In preclinical studies, animals are immunized with an antigen such as KLH while being dosed with a drug candidate. The levels of anti-KLH IgM and IgG are determined in serum collected at 7 or ~21 days and compared with those in a control group that was not exposed to the drug. A decrease in anti-KLH levels in the treatment group provides evidence of immunosuppression. A list of publications citing the use of our kits can be found at the bottom of this page.
Orders may be placed by phone, fax, email, or via our online shop.
For technical advice please email us at [email protected]. All kits listed below are manufactured by Life Diagnostics, Inc. All kits are manufactured by Life Diagnostics, Inc.
| Anti-KLH IgG & IgM ELISA Kits |
|||
| ELISA | Instructions | Cat. # |
Price |
| Mouse Anti-KLH IgG | Insert | KLHG-1 | $575 |
| Mouse Anti-KLH IgM | Insert | KLHM-1 | $575 |
| Rat Anti-KLH IgG | Insert | KLHG-2 | $575 |
| Rat Anti-KLH IgM | Insert | KLHM-2 | $575 |
| Monkey Anti-KLH IgG1 | Insert | KLHG1-3 | $600 |
| Monkey Anti-KLH IgG (For Export)* | Insert | KLHG-3-INT | $600 |
| Monkey Anti-KLH IgM | Insert | KLHM-3 | $600 |
| Monkey Anti-KLH IgM (For Export)* | Insert | KLHM-3-INT | $600 |
| Guinea Pig Anti-KLH IgG | Insert | KLHG-7 | $575 |
| Human Anti-KLH IgG | Insert | KLHG-20 | $600 |
| Human Anti-KLH IgM | Insert | KLHM-20 | $600 |
| *Kits KLHM-3-INT and KLHG-3-INT use standard material of non monkey origin, allowing for exportation without CITES documentation. | |||
|
Anti-Sheep Red Blood Cell IgG & IgM ELISA Kits |
|||
| ELISA | Instructions | Cat. # | Price |
| Mouse Anti-SRBC IgG | Insert | SRBCG-1 | $575 |
| Mouse Anti-SRBC IgM | Insert | SRBCM-1 | $575 |
| Rat Anti-SRBC IgG | Insert | SRBCG-2 | $575 |
| Rat Anti-SRBC IgM | Insert | SRBCM-2 | $575 |
| Chicken Anti-SRBC IgG | Insert | SRBCG-5 | $550 |
| Chicken Anti-SRBC IgM | Insert | SRBCM-5 | $550 |
|
Anti-Tetanus Toxoid IgG & IgM ELISA Kits |
|||
| ELISA | Kit Instructions | Cat. # |
Price |
| Rat Anti-Tetanus Toxoid IgG | Insert | TTG-2 | $575 |
| Rat Anti-Tetanus Toxoid IgM | Insert | TTM-2 | $575 |
| Monkey Anti-Tetanus Toxoid IgG1 | Insert | TTG1-3 | $600 |
| Monkey Anti-Tetanus Toxoid IgM | Insert | TTM-3 | $600 |
| Anti-DNP IgG & IgM ELISA Kits |
|||
| ELISA | Kit Instructions | Cat. # | Price |
| Mouse Anti-DNP IgG | Insert | DNPG-1 | $550 |
| Mouse Anti-DNP IgM | Insert | DNPM-1 | $550 |
| Rat Anti-DNP IgG | Insert | DNPG-2 | $550 |
| Rat Anti-DNP IgM | Insert | DNPM-2 | $550 |
| Anti-TNP IgG & IgM ELISA Kits |
|||
| ELISA |
Kit Instructions | Cat. # | Price |
| Mouse Anti-TNP IgG | Insert | TNPG-1 | $550 |
| Mouse Anti-TNP IgM | Insert | TNPM-1 | $550 |
Our rat and monkey anti-KLH IgG ELISA kits have recently been evaluated and gained unqualified approval in a respected independent laboratory (Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. CIT, Evreux, France. Armelle N. Guyen, Sandrine Arthaud, Phillipe Ancian and Roy Forster. Toxicology Letters, Volume 180, Supplement 1, 5 October 2008, Page S211).
Publications describing the use of LDI Immunotoxicity kits
- Lo J, et al. Immature dendritic cell therapy confers durable immune modulation in an antigen-dependent and antigen-independent manner in nonobese diabetic mice. Journal of Immunology Research. Volume 2018 Article ID 5463879 (2108) [Mouse anti-KLH IgG & IgM]
- Ksiazek A and Konarzewski M. Heat dissipation does not suppress an immune response in laboratory mice divergently selected for basal metabolic rate (BMR). Journal of Experimental Biology. 219:1542-1551 (2016) [Mouse anti-KLH IgG & IgM]
- Tan X, et al. Immunotoxicological evaluation of genetically modified rice expressing Cry1Ab/AcProtein (TT51-1) by a 6-month feeding study on cynomolgus monkeys. PLoSONE 11(9):e0163879.doi:10.1371/journal.pone.0163879 (2016) [Monkey anti-KLH IgG & IgM]
- Ma H, Wan S, and Xia C-Q. Immunosuppressive CD11b+Ly6Chi monocytes in pristane-induced lupus mouse model. Journal of Leukocyte Biology 99:1121 (2016). [Mouse anti-KLH IgG]
- Nixon AE, et al. Fully human monoclonal antibody inhibitors of the neonatal Fc receptor reduce circulating IgG in non-human primates. Frontiers in Immunology. Volume 6, Article 176. (2015) [Monkey anti-tetanus toxoid & anti-KLH IgG & IgM]
- Bernett MJ, et al. Immune suppression in cynomolgus monkeys by XPro9523. (2013) https://doi.org/10.4161/mabs.23976 [Monkey anti-KLH IgG & IgM]
- Guyen AN, Arthaud S, Ancian P and Foster R. Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. Toxicology Letters (Abstracts) 180S: S211/I11 (2008)
- Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, Kim CH and Taparowsky EJ. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J. Exp. Med. 207(5):933-942 (2011) [Mouse anti-SRBC IgG & IgM] http://jem.rupress.org/content/207/5/933.full.pdf+html
- Schaffer BS, Grayson MH and Wortham JM, et al. Immune competency of a hairless mouse strain for improved preclinical studies in genetically engineered mice. Mol. Cancer Ther. 9:2354-2364 (2010) [Mouse anti-KLH IgG & IgM] http://mct.aacrjournals.org/content/9/8/2354.full.pdf+html
- Efanov A, Zanesi N, NazaryanN, Santanam U, Palmarchuck A Croce CM and Pekarsky Y. CD5+CD23+ leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation. Leukemia 24:970-975 (2010) [Mouse anti-SRBC IgM]
- Nain S and Smits JEG. Pathological, immunological and biochemical markers of subchronic arsenic toxicity in rats. Environmental Toxicology. (2010) http://onlinelibrary.wiley.com/doi/10.1002/tox.20635/abstract [Rat anti-KLH IgG & IgM]
- Zhou X, Xiang Y, Ding, X and Garrard WT. Anew hypersensitive site, HS10, and the enhancers, E3′ and Ed, differentially regulate Igk gene expression. J. Immunol. 188:2722-2732 (2012) [Mouse anti-KLH IgG & IgM]
- Sah S and Smits J. Dietary selenium fortification: a potential solution to chronic arsenic toxicity. Toxicological & Environmental Chemistry. 94(7): 1453-1465 (2012) [Rat anti-KLH IgG]
- Anderson SE, Franko J, Anderson KL, Munson AE, Lukomska E, Meade BJ. Immunotoxicity and allergic potential induced by topical application of dimethyl carbonate (DMC) in a murine model. J Immunotoxicol. [Epub ahead of print] (2012) [Mouse anti-SRBC IgM]
- Aulí M, et al. Multiparametric immunotoxicity screening in mice during early drug development. Toxicol Lett. [Epub ahead of print] (2012) [Mouse anti-KLH IgM]
- Sah S and Smits J. Treating chronic arsenic toxicity with high selenium lentil diets. Toxicol Appl Pharmacol. doi: 10.1016/j.taap.2013.06.008. (2013) [Rat anti-KLH IgG]